Anti-ARAP1 Purified

ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.

The antibody ARAP1-2 reacts with C-terminal part of ARAP1 (intracellular epitope), a 160 kDa adaptor protein.

RUO

Mouse

Human

His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1

ARAP1

IgG1

ARAP1-2

Western blotting: Recommended dilution: 2 μg/mL

IP,WB,ICC

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar13(3):423-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18165900?ordin,

*Miura K, Jacques KM, Stauffer S, Kubosaki A, Zhu K, Hirsch DS, Resau J, Zheng Y, Randazzo PA: ARAP1: a point of convergence for Arf and Rho signaling. Mol Cell. 2002 Jan9(1):109-19., URL: https://www.ncbi.nlm.nih.gov/pubmed/11804590?ordin,*Guo DF, Chenier I, Tardif V, Orlov SN, Inagami T: Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. Biochem Biophys Res Commun. 2003 Oct 31310(4):1254-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/14559250?ordin,

Store at 2-8°C. Do not freeze.