Anti-Hu CD370 APC

CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.

The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

RUO

Mouse

Human

RBL-2H3 cells expressing human CLEC9A fused to an HA epitope

CD370

IgG2a

8F9

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

FC

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Sancho D, Mour227o-Sá D, Joffre OP, Schulz O, Rogers NC, Pennington DJ, Carlyle JR, Reis e Sousa C: Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008 Jun118(6):2098-110. doi: 10.1172/JCI34584., URL: http://www.ncbi.nlm.nih.gov/pubmed/18497879 ,*Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, H229kansson UK, Moita LF, Agace WW, Bonnet D, Reis e Sousa C: DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues. Blood. 2012 Jun 21119(25):6052-62., URL: http://www.ncbi.nlm.nih.gov/pubmed/22442345 ,*Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S: Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9209(4):653-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/22430490,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.