MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2)

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Mouse

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH was used as the immunogen for this MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2).

Monoclonal

IgG1

WB, IHC-P, ICC

Ascites

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

After reconstitution, the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) is available for research use only.