Anti-BCL6 APC

BCL6 is a zinc finger-containing transcription factor, which acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. On B cells, BCL6 induces proliferation, antibody class switching and affinity maturation, whereas it inhibits their differentiation into plasma cells. On T cells, BCL6 induces their differentiation into T-FH cells.

The mouse monoclonal antibody IG191E/A8 recognizes BCL6, a transcriptional repressor serving as a master regulator of germinal center reaction.

RUO

Mouse

Mouse,Human

pCDNA3 plasmid containing full-length sequence of human BCL6

BCL6

IgG1

IG191E/A8

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests. Intracellular staining.

FC

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Jain S, Stock A, Macian F, Putterman C: A Distinct T follicular helper cell subset infiltrates the brain in murine neuropsychiatric lupus. Front Immunol. 2018 Mar 139:487., URL: https://pubmed.ncbi.nlm.nih.gov/29593732/,*Hoser D, Schön C, Loddenkemper C, Lohneis P, Kühl AA, Sommermann T, Blankenstein T, Willimsky G: Oncogene-specific T cells fail to eradicate lymphoma-initiating B cells in mice. Blood. 2018 Aug 30132(9):924-934., URL: https://pubmed.ncbi.nlm.nih.gov/30002144/,*Tan HX, Esterbauer R, Vanderven HA, Juno JA, Kent SJ, Wheatley AK: Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Front Immunol. 2019 Mar 2910:611., URL: https://www.ncbi.nlm.nih.gov/pubmed/30984186,*Jiang W, Wragg KM, Tan HX, Kelly HG, Wheatley AK, Kent SJ, Juno JA: Identification of murine antigen-specific T follicular helper cells using an activation-induced marker assay. J Immunol Methods. 2019 Apr467:48-57., URL: https://pubmed.ncbi.nlm.nih.gov/30802449/,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.