Micro spin cup

Specifications:
	Product is designed to prepare plasmid DNA (small scale) from E.coli cells
	Product contents are micro spin cups, RNAse A, ready-to-use buffers and protocol
	Very high DNA capacity (up to 50 µg plasmid DNA per micro-spin cup)
	Ultra quick procedure using micro spin cups
	No chromosomal DNA contamination
	No ethanol or isopropanol precipitation
	No phenol/chloroform extraction
	No organic or toxic reagents
	Excellent reproducibility
	Culture volumes up to 5ml
	Total procedure is < 25min
	Plasmid sizes are up to 20.000bp
	Plasmid recovery is 90% - 95% on average
	Plasmid quality matches CsCl purified DNA
	A260/280 ratio is > 1.8
	DNA elution in TE buffer or equivalent low-salt buffer or simply water
	Micro-spin cups are adaptable to vacuum manifold (see protocol links below)
Applications:
	Fluorescent sequencing
	Ligation
	Restriction analysis
	PCR template preparation
	Labeling
	All other enzymatic reactions

A number of twelve plasmid preparations were carried out simultaneously from twelve separate 1 ml E.coli cultures using the JETQUICK Plasmid Miniprep Kit. The plasmid was pT7T3 19U. Aliquots were run on a 1% agarose ge

                                                              LFU-Unit                   Midi column                  Maxi column

Advantages:
	Very convenient procedure - much simpler and shorter than any competitor's protocol
	Great time-saving compared to standard procedure
	Newly designed FAST Midi columns much faster than any comparable product
	Larger culture volumes can be processed on the new FAST Midi columns
	No centrifugation necessary to clear bacterial lysate
	Lysate clarification integrated into column procedure
	Only ONE column wash for the new FAST Midi and Maxi columns
	No clogging of the lysate filter unit
	Much less susceptible against overloading than competitor's products
JETSTAR specifications:
	Product is designed to prepare plasmid DNA (medium and large scale) from E.colicells
	Product contents are gravity fast flow columns, RNase A, ready-to-use buffers and protocol
	JETSTAR / LFU preparations are available in Midi and Maxi format
	JETSTAR is able to prepare cosmid DNA and BAC DNA as well
	No chromosomal DNA contamination
	No phenol/chloroform extraction
	No organic or toxic reagents
	Excellent reproducibility
	Total procedure in 30 - 40 min
	Plasmid DNA is ultrapure (better than 2 x CsCl)
	Plasmid DNA recovery is 90% - 95% on average
	Plasmid DNA of all sizes can be prepared
	Plasmid DNA yields are up to 200 µg (Midi) and up to 850 µg (Maxi)
	A260/280 ratio is > 1.85
	Endotoxin level is < 0.01 EU/µg plasmid DNA
Applications for plasmid and cosmid DNA
	Transfection
	Manual and automated sequencing, including capillary sequencing
	Microinjection
	In vitro transcription
	Restriction analysis, ligation, labeling etc.
	Transfection of endotoxin-sensitive cells (Endo Free)
	Gene therapy applications