Anti-Hu SUSD2 APC

SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.

The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.

RUO

Mouse

Human

WERI-RB-1 retinoblastoma cells

SUSD2

IgG1

W5C5

Flow cytometry: Recommended dilution: 1-4 µg/mL

FC

0.1 mg/mL

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Bredenkamp N, Stirparo GG, Nichols J, Smith A, Guo G: The cell-surface marker sushi containing domain 2 facilitates establishment of human naive pluripotent stem cells. Stem Cell Reports. 2019 Jun 1112(6):1212-1222., URL: https://pubmed.ncbi.nlm.nih.gov/31031191/,*Murakami K, Lee YH, Lucas ES, Chan YW, Durairaj RP, Takeda S, Moore JD, Tan BK, Quenby S, Chan JK, Gargett CE, Brosens JJ: Decidualization induces a secretome switch in perivascular niche cells of the human endometrium. Endocrinology. 2014 Nov155(11):4542-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/25116707,*Lee BJ, Kang DW, Park HY, Song JS, Kim JM, Jang JY, Lee JC, Wang SG, Jung JS, Shin SC: Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2). Cell Physiol Biochem. 201638(1):83-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/26741828,*Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun1106:262-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17395729,*Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M, Lengerke C, Maurer A, Kalbacher H, Bühring HJ: Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2. Stem Cells Dev. 2013 Jul 122(13):1944-54., URL: https://www.ncbi.nlm.nih.gov/pubmed/23406305,*Ulrich D, Tan KS, Deane J, Schwab K, Cheong A, Rosamilia A, Gargett CE: Mesenchymal stem/stromal cells in post-menopausal endometrium. Hum Reprod. 2014 Sep29(9):1895-905., URL: https://www.ncbi.nlm.nih.gov/pubmed/24964924,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.