Anti-Hu CD235a Biotin

CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.

The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

RUO

Mouse

Rat,Human

Membrane preparation from splenic hairy cell leukemia

CD235a

IgG1

JC159

Flow cytometry: Recommended dilution: 1-4 μg/mL.

IHC-P,IHC-F,FC

1 mg/mL

Biotin

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

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*Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 199413: 401., URL: https://pubmed.ncbi.nlm.nih.gov/8069185/,*Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 200852(2):69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18380804?ordin,*Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996 24: 597., URL: https://www.ncbi.nlm.nih.gov/pubmed/8605964?dopt=A,*Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing 1990., URL: ,*Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press 1994., URL: ,

Store at 2-8°C. Do not freeze.