TNF alpha Antibody

This mAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

P01375

Mouse

Recombinant full-length human protein was used as the immunogen for the TNF-alpha antibody.

Monoclonal

IgM κ

FACS, IF, IHC-P

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Store the TNF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This TNF-alpha antibody is available for research use only.