Cyclin B1 Antibody / CCNB1

This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.

Mouse

Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Monoclonal

IgG1 κ

FACS, IF, IP

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This Cyclin B1 antibody is available for research use only.