T (Testosterone) ELISA Kit

T

Universal

Competitive-ELISA

ELISA

Signal Transduction;Signal Transduction;Epigenetics and Nuclear Signaling;Developmental Biology

Colormetric

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal T. During the reaction, Universal T in the sample or standard competes with a fixed amount of Universal T on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal T. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal T in tested samples can be calculated by comparing the OD of the samples to the standard curve.

2 h 30 min

0.31-20 ng/mL

0.17 ng/mL

Ice bag

2-8°C/-20°C, 12 months