Anti-Hu CD11b APC

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

The mouse monoclonal antibody ICRF44 recognizes an extracellular epitope of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.

RUO

Mouse

Human,Non-human primates

Rheumatoid synovial cells and human monocytes.

CD11b

IgG1

ICRF44

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

FC

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

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*Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 195(3):911-20., URL: https://pubmed.ncbi.nlm.nih.gov/10648403/,*Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15119(3-4):287-98., URL: https://pubmed.ncbi.nlm.nih.gov/17628696/,*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 200743(8):597-606., URL: https://pubmed.ncbi.nlm.nih.gov/17895634/,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.