Anti-Hu CD340 FITC

The oncoprotein ErbB2/HER2 (human epidermal growth factor receptor 2), also known as Neu or CD340, is a 185 kDa transmembrane tyrosine kinase of cell surface growth factor receptor family. It is present in a wide variety cell types of normal human fetal and adult tissues and is frequently overexpressed in human carcinomas (e.g. in 20-30% cases of breast cancer cells). Activation of ErbB2 triggers intracellular signalling events, which are essential for cell growth and differentiation. In the last 20 years ErbB2 antigen has become very important marker and therapy target in patient care.

The mouse monoclonal antibody 24D2 recognizes an extracellular epitope of human CD340 (HER-2), a type I transmembrane protein expressed by mesenchymal stem cells, B-lymphoblastic leukemia cells, subsets of C-ALL blasts, and and various types of carcinomas.

RUO

Mouse

Human

NIH-3T3/huCD340 cells

CD340

IgG1

24D2

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.

FC

FITC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Bühring HJ, Sures I, Jallal B, Weiss FU, Busch FW, Ludwig WD, Handgretinger R, Waller HD, Ullrich A: The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia. Blood. 1995 Sep 186(5):1916-23., URL: https://pubmed.ncbi.nlm.nih.gov/7544646/,*Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun1106:262-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17395729,*Tran T, Diniz MO, Dransart E, Gey A, Merillon N, Lone YC, Godefroy S, Sibley C, Ferreira LC, Medioni J, Oudard S, Johannes L, Tartour E: A Therapeutic Her2/neu Vaccine Targeting Dendritic Cells Preferentially Inhibits the Growth of Low Her2/neu-Expressing Tumor in HLA-A2 Transgenic Mice. Clin Cancer Res. 2016 Aug 1522(16):4133-44. , URL: https://www.ncbi.nlm.nih.gov/pubmed/27006496,*Bailey TA, Luan H, Tom E, Bielecki TA, Mohapatra B, Ahmad G, George M, Kelly DL, Natarajan A, Raja SM, Band V, Band H: A kinase inhibitor screen reveals protein kinase C-dependent endocytic recycling of ErbB2 in breast cancer cells. J Biol Chem. 2014 Oct 31289(44):30443-58., URL: https://pubmed.ncbi.nlm.nih.gov/25225290/,

*Arora P, Cuevas BD, Russo A, Johnson GL, Trejo J: Persistent transactivation of EGFR and ErbB2/HER2 by protease-activated receptor-1 promotes breast carcinoma cell invasion. Oncogene. 2008 Mar 31, URL: https://www.ncbi.nlm.nih.gov/pubmed/18372913?ordin,*Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Signaling through ShcA is required for TGF-{beta} and Neu/ErbB-2 induced breast cancer cell motility and invasion. Mol Cell Biol. 2008 Mar 10, URL: https://www.ncbi.nlm.nih.gov/pubmed/18332126?ordin,*Yuan W, Wang W, Cui B, Su T, Ge Y, Jiang L, Zhou W, Ning G: Overexpression of ERBB-2 was more frequently detected in malignant than benign pheochromocytomas by multiplex ligation-dependent probe amplification and immunohistochemistry. Endocr Relat Cancer. 2008 Mar15(1):343-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/18310300?ordin,*Hicks DG, Kulkarni S: HER2+ breast cancer: review of biologic relevance and optimal use of diagnostic tools. Am J Clin Pathol. 2008 Feb129(2):263-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/18208807?ordin,*Dowsett M, Hanna WM, Kockx M, Penault-Llorca F, Rüschoff J, Gutjahr T, Habben K, van de Vijver MJ: Standardization of HER2 testing: results of an international proficiency-testing ring study. Mod Pathol. 2007 May20(5):584-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/17396141?ordin,*Koeppen HK, Wright BD, Burt AD, Quirke P, McNicol AM, Dybdal NO, Sliwkowski MX, Hillan KJ: Overexpression of HER2/neu in solid tumours: an immunohistochemical survey. Histopathology. 2001 Feb38(2):96-104., URL: https://www.ncbi.nlm.nih.gov/pubmed/11207822?dopt=,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.