Anti-Ms CD49e FITC

CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.

The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

RUO

Rat

Mouse

C57BL/6 x A/J)F1 murine mast cell line

CD49e

IgG2a κ

5H10-27 (MFR5)

Flow cytometry: Recommended dilution: 1-4 μg/mL.

FC

0.5 mg/mL

FITC

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*van der Loo JC, Xiao X, McMillin D, Hashino K, Kato I, Williams DA: VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin. J Clin Invest. 1998 Sep 1102(5):1051-61., URL: https://pubmed.ncbi.nlm.nih.gov/9727075/,*Sampaio AL, Zahn G, Leoni G, Vossmeyer D, Christner C, Marshall JF, Perretti M: Inflammation-dependent alpha 5 beta 1 (very late antigen-5) expression on leukocytes reveals a functional role for this integrin in acute peritonitis. J Leukoc Biol. 2010 May87(5):877-84., URL: https://pubmed.ncbi.nlm.nih.gov/20097849/,*Plett PA, Frankovitz SM, Orschell CM: Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation. Blood. 2003 Sep 15102(6):2285-91., URL: http://www.ncbi.nlm.nih.gov/pubmed/12775569 ,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.