This Human Alpha-2-macroglobulin-like protein 1 (A2ML1) ELISA Kit employs a two-site sandwich ELISA to quantitate A2ML1.

• Additional information:The alpha-macroglobulin (AM) superfamily of proteins contains both complement components and protease inhibitors, including A2M and A2ML1. AM proteins display a unique trap mechanism of inhibition, by which the AM inhibitor undergoes a major conformational change upon its cleavage by a protease, thus trapping the protease and blocking it from subsequent substrate binding. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates. Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
• Storage and shipping:Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.Shipping:Gel pack with blue ice.
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.