A competitive Inhibition ELISA kit for detection of Orexin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

• Additional information:A monoclonal antibody specific to Orexin (OX) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Orexin (OX) and unlabeled Orexin (OX) (Standards or samples) with the pre-coated antibody specific to Orexin (OX). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Orexin (OX) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Orexin (OX) in the sample.
• Storage and shipping:The Human OX ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
• Notes:This kit is intended for research use only. Do not use for diagnostics purposes.