Quantitative sandwich ELISA for measuring Human Peptidoglycan recognition protein L (PGRP-L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:The deduced 576-amino acid precursor protein contains an N-terminal signal peptide, followed by 2 transmembrane segments and an extracellular C terminus containing PGRP domains III, II, and I. PGRPL shares 40%, 33%, and 32% identity with PGRPS, PGRPI-alpha, and PGRPI-beta, respectively. RNA dot blot analysis detected strong PGRPL expression in adult liver and weak expression in fetal liver. Northern blot analysis detected 2.1- and 0.8-kb transcripts expressed in adult and fetal liver. PCR also detected low expression in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach, and testis. Transiently transfected COS-7 and human embryonic kidney cells expressed PGRPL as a Triton X-100-soluble membrane protein with an apparent molecular mass of 65 kD.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.