A competitive Inhibition ELISA kit for detection of Proinsulin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

• Additional information:A monoclonal antibody specific to Proinsulin (PI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Proinsulin (PI) and unlabeled Proinsulin (PI) (Standards or samples) with the pre-coated antibody specific to Proinsulin (PI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Proinsulin (PI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Proinsulin (PI) in the sample.
• Storage and shipping:The Human PI ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
• Notes:This kit is intended for research use only. Do not use for diagnostics purposes.