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ELISA kit for Human PK (Pyruvate Kinase, Liver And RBC)

CAT:ELK1922Size:1 plate of 96 wellsPrice:Ask for quotation
Availability:
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Shipping:
This product ships without dry ice.



ELISA kit for Human PK (Pyruvate Kinase, Liver And RBC)

Product Description:

A sandwich ELISA kit for detection of Pyruvate Kinase, Liver And RBC from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Specifications:

Species reactivity: Human

Sensitivity: 17pg/mL

Detection range: 39.06-2500pg/mL

Standard solution concentration: 2500pg/mL

ELISA type: Sandwich

Assay length: 3h

Storage and shipping:

The Human PK ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

Additional informations:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Liver And RBC (PK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Liver And RBC (PK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Liver And RBC (PK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &plusmn

10nm. The concentration of Pyruvate Kinase, Liver And RBC (PK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Notes:

This kit is intended for research use only. Do not use for diagnostics purposes.