A sandwich ELISA kit for detection of Semaphorin 4B from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

• Additional information:
  
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4B (SEMA4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4B (SEMA4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4B (SEMA4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
  • 10nm. The concentration of Semaphorin 4B (SEMA4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Storage and shipping:The Human SEMA4B ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
• Notes:This kit is intended for research use only. Do not use for diagnostics purposes.