Quantitative sandwich ELISA for measuring Human Wilms tumor protein (WT1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:Wilms tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a subset of patients with Wilms' tumor, the gene's namesake. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.The WT1 protein has been found to bind a host of cellular factors, e.g. p53, a known tumor suppressor.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.