This Human Zinc finger BED domain-containing protein 3 (ZBED3) ELISA Kit employs a two-site sandwich ELISA to quantitate ZBED3.

• Additional information:Zbed3 contains a PPPPSPT motif, which is crucial to its binding to Axin. The Ser and Thr residues in the motif appear to be also phosphorylated by glycogen synthase kinase 3? (GSK3?) and the CKI family kinases, as GSK3? and CKI? could enhance the interaction of Zbed3 with Axin. Expressing Zbed3, but not these mutants, led to inhibition of GSK3?-mediated ?-catenin phosphorylation, cytoplasmic ?-catenin accumulation, and activation of lymphoid enhancer binding factor-1-dependent reporter gene transcription. Furthermore, knockdown of Zbed3 with RNA interference attenuated Wnt-induced ?-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and the Wnt target gene expression. Zbed3 is a novel Axin-binding protein that is involved in Wnt/?-catenin signaling modulation.
• Storage and shipping:Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.Shipping:Gel pack with blue ice.
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.