Quantitative sandwich ELISA for measuring Mouse Secretagogin (SCGN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:SCGN contains 6 tandem repeats of the EF-hand Ca(2+)-binding domain, with a loop at all 6 putative Ca(2+)-binding sites, and shares significant similarity with calbindin and calretinin. Much lower expression of only the smaller transcript was detected in adrenal medulla, stomach, thyroid, colon, and small intestine. Immunohistochemical analysis showed SCGN expression restricted to the islet of Langerhans in pancreas, in C cells near thyroid follicles, and at sites typically occupied by neuroendocrine cells in all other tissues examined. Cell fractionation and confocal imaging of transfected rat insulinoma cells detected Scgn predominantly in the cytosolic fraction, with much less Scgn in nuclei. SCGN was expressed in a reticular pattern in transfected COS cells.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.