A sandwich ELISA kit for detection of Claudin 1 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

• Additional information:
  
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 1 (CLDN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 1 (CLDN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 1 (CLDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
  • 10nm. The concentration of Claudin 1 (CLDN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Storage and shipping:The Rat CLDN1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
• Notes:This kit is intended for research use only. Do not use for diagnostics purposes.