Quantitative sandwich ELISA for measuring Rat Methionine adenosyltransferase 2 subunit beta (MAT2B) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:
  
  • Methionine adenosyltransferase (MAT
  • EC 2.5.1.6) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet) from methionine and ATP. MAT II is a broadly expressed MAT consisting of catalytic alpha and noncatalytic beta subunits encoded by MAT2A) and MAT2B, respectively.LeGros et al. (2000) designed degenerate PCR primers to tryptic peptide sequence of MAT2B purified from human lymphocytes. They amplified a partial cDNA from Jurkat cells and extended the cDNA using 3-prime and 5-prime RACE. A full-length MAT2B cDNA amplified from normal human peripheral blood mononuclear cell RNA encodes a 334-amino acid protein with a molecular mass of 38 kD.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.