A competitive ELISA kit for quantitative measurement of Rat VIP (Vasoactive Intestinal Peptide) in samples from Serum, Plasma, Cell supernatant

• Additional information:Gentaur's VIP ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat VIP. During the reaction, Rat VIP in the sample or standard competes with a fixed amount of Rat VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat VIP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat VIP in the samples is then determined by comparing the OD of the samples to the standard curve.
• Storage and shipping:The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.
• Notes:This Rat VIP ELISA kit is intended for research use only. Do not use for human or animal diagnostics.