The PRMT3 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT3_x000D_activity for screening and profiling applications. The key to the PRMT3 Direct Activity Assay Kit is a highly_x000D_specific antibody that recognizes methylated substrate. With this kit, only three simple_x000D_steps on a microtiter plate are required for methyltransferase detection. First,_x000D_Sadenosylmethionine is incubated with a sample containing assay buffer and_x000D_methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the_x000D_plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a_x000D_chemiluminescence reader.