The SMYD4 Chemiluminescent Assay Kit is designed to measure SMYD4_x000D_activity for screening and profiling applications. The SMYD4 Chemiluminescent Assay Kit comes_x000D_in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against_x000D_methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine,_x000D_methyltransferase assay buffer, and purified SMYD4 enzyme for 96 enzyme_x000D_reactions. The key to the SMYD4 Chemiluminescent Activity Assay Kit is a highly specific_x000D_antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps_x000D_are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a_x000D_sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added._x000D_Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the_x000D_HRP substrate to produce chemiluminescence that can then be measured using a_x000D_chemiluminescence reader.