The UTX Chemiluminescent Assay Kit is designed to measure UTX activity for screening and profiling applications. UTX, also known as KDM6A, is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 on histone H3 (H3K27me2/3).The key to the UTX Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, UTX enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.