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EndoG Antibody [7F2G10]
EndoG Antibody [7F2G10]
Product Description:EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in <i>C. elegans</i>. EndoG also cleaves DNA in vitro.
Research area: Apoptosis
Host species: Mouse
Species reactivity: Human, Rat
Homology: N/A
Immunogen: Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
Tested Applications: E, WB
Applications: EndoG monoclonal monoclonal antibody can be used for detection of EndoG by Western blot at 5 - 10 μ
g/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity: N/A
Positive control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive control 2: Cat. No. 95-103 - EndoG Recombinant Protein
Positive control 3: N/A
Positive control 4: N/A
Positive control 5: N/A
Positive control 6: N/A
Predicted molecular weight: N/A
MW explanation: N/A
Validation: N/A
Isoforms: N/A
Purification: EndoG Monoclonal Antibody is Protein A purified.
Clonality: Monoclonal
Clone: 7F2G10
Isotype: IgG1
Conjugate: Unconjugated
Physical state: Liquid
Buffer: EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
NCBI official symbol: ENDOG
Accession #: Q14249
Protein GI #: 24638471
NCBI gene ID#: 2021
NCBI official full name: endonuclease G
NCBI organism: Homo sapiens
SWISSPROT #: Q14249
EndoG monoclonal antibody can be stored at -20˚C, stable for one year.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Optimal dilutions for each application to be determined by the researcher.
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