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ELISA kit for Horse TGFb1 (Transforming Growth Factor Beta 1)
ELISA kit for Horse TGFb1 (Transforming Growth Factor Beta 1)
Product Description:A sandwich ELISA kit for detection of Transforming Growth Factor Beta 1 from Horse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Species reactivity: Horse
Sensitivity: 28pg/mL
Detection range: 78-5000pg/mL
Standard solution concentration: 5000pg/mL
ELISA type: Sandwich
Assay length: 3h
The Horse TGFb1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGF?1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGF?1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGF?1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
10nm. The concentration of Transforming Growth Factor Beta 1 (TGF?1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
This kit is intended for research use only. Do not use for diagnostics purposes.
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