Anti-Hu CD52 PE

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

The mouse monoclonal antibody 4C8 recognizes CD52, a GPI-anchored glycopeptide expressed on the surface of lymphocytes, macrophages, epithelial cells of male reproductive tract, and mature sperms.

RUO

Mouse

Human

human lymphocytes

CD52

IgG3

4C8

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

FC

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Masuyama J, Kaga S, Kano S, Minota S: A novel costimulation pathway via the 4C8 antigen for the induction of CD4+ regulatory T cells. J Immunol. 2002 Oct 1169(7):3710-6., URL: https://pubmed.ncbi.nlm.nih.gov/12244164/?from_term=masuyama+kaga+&from_pos=4
,*Masuyama J, Yoshio T, Suzuki K, Kitagawa S, Iwamoto M, Kamimura T, Hirata D, Takeda A, Kano S, Minota S: Characterization of the 4C8 antigen involved in transendothelial migration of CD26(hi) T cells after tight adhesion to human umbilical vein endothelial cell monolayers. J Exp Med. 1999 Mar 15189(6):979-90., URL: https://pubmed.ncbi.nlm.nih.gov/10075981/?from_term=masuyama+yoshio+suzuki&from_schema=all&from_pos=3,*Toh BH, Kyaw T, Tipping P, Bobik A: Immune Regulation by CD52-expressing CD4 T Cells. Cell Mol Immunol. 2013 Sep10(5):379-82., URL: https://pubmed.ncbi.nlm.nih.gov/23934027/?from_term=tipping+p+bobik+a+kyaw&from_page=2&from_pos=6,*Watanabe T, Masuyama J, Sohma Y, Inazawa H, Horie K, Kojima K, Uemura Y, Aoki Y, Kaga S, Minota S, Tanaka T, Yamaguchi Y, Kobayashi T, Serizawa I: CD52 is a novel costimulatory molecule for induction of CD4+ regulatory T cells. Clin Immunol. 2006 Sep120(3):247-59., URL: https://pubmed.ncbi.nlm.nih.gov/16797237/?from_term=watanabe+inazawa&from_page=2&from_pos=7,

*Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15274(42):29862-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/10514467?ordin,*Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17270(11):6088-99. , URL: https://www.ncbi.nlm.nih.gov/pubmed/7890742?ordina,*Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan7(1):69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/7718516?ordina,*Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 200763:103-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/17566265?ordin,*Domagała A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr7(2):325-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/11257744?ordin,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.